Figure 6.

Influence of PXR on interaction between PPARα and PGC1α. (A,B) HepG2 cells were transfected with pGL4.31, PGC1α-LXXLL-pFN11A, an expression plasmid for hPXR (A) or hPPARα (B) fused to VP16 TAD. (C) An expression plasmid for hPXR or hPXR-ΔAF2 (1 or 10 ng) was co-expressed as in Figure 6b. Twenty-four hours after the transfection, the cells were treated with vehicle (Veh, 0.2% DMSO), 100 μM bezafibrate (BZF), and/or 10 μM rifampicin (Rif) for 24 h. Relative reporter activity was determined. Data are shown as the mean ± S.D. (n = 4). Differences between the indicated combinations were assessed by Dunnett’s test (* p < 0.05); NS, not significant.