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L1-KO mice have increased mRNA levels of genes involved in adaptive thermogenesis and fatty acid oxidation in BAT: (A) plasma concentrations of bile acids analyzed by UPLC–MS/MS in WT and L1-KO mice after 6 weeks of HDF feeding (n = 8); (B) relative mRNA levels in the interscapular BAT measured by qPCR with individual total RNA samples in each group (n = 5). For this study, 18S RNA was used as an internal invariant control. * p < 0.05.
