Table 3.

Primers and PCR conditions used in this study.

	Primer Sequences	No. of PCR Cycles and PCR Conditions	Size (bp)	Ref
Lactobacillus	F: 5′-GACTTGGTTGAAATGGAAGT-3′ R: 5′-TCAGTGGTGTGGAAGTAGAA-3′	40 (35 s at 95 °C, 35 s at 54 °C, 35 s at 72 °C) 40 s at 72 °C	572	[31]
16 s	F:5′AGAGTTTGATCCTGGCTCAG-3′ R: 5′-GGTTACCTTGTTACGACT-3′	30 (60 s at 94 °C, 60 s at 50 °C, 60 s at 72 °C) 7 min at 72 °C	1500	[32]
lepA	F: 5′-GGDCACGTRGAYTTYTCWTAYGA-3′ R:5′-GCATAVCCYTTNGTDRAWGACTT-3′	35 (60 s at 94 °C, 75 s at 50 °C, 60 s at 72 °C) 7 min at 72 °C	1163	In this study
Lim. fermentum	F: 5′-AATACCGCATTA CAACTTTG -3′ R: 5′-GGTTAAATACCCTCAACGTA -3′	35 (60 s at 94 °C, 60 s at 50 °C, 60 s at 72 °C) 7 min at 72 °C	337	[33]
L. reuteri	F: 5′-CAGACAATCTTTGATTGTTTAG-3′ R: 5′-GCTTGTTGGTTTGGGCTCTTC-3′	40 (30 s at 95 °C, 60 s at 60 °C, 120 s at 2 °C) 2 min at 72 °C	300	[34]