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Immunofluorescence reaction for PSA-FITC in ejaculated (A), capacitated (B) and acrosome-reacted (C) spermatozoa. Preservation of the integrity of the acrosome is observed in a large part of the ejaculated and capacitated spermatozoa, while in acrosome-reacted spermatozoa only the equatorial segment is marked. Scale bar = 5 μm.
