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Central Composite Design (CCD) obtained by RSM, with two independent variables (time (t) in days and temperature (T) in °C), three center points and two-star points. The design was replicated.
| Time (Days) | Temperature (°C) |
|---|---|
| 2 | 17 |
| 3 | 21 |
| 3 | 21 |
| 2 | 25 |
| 4 | 25 |
| 3 | 21 |
| 1 | 21 |
| 5 | 21 |
| 4 | 17 |
| 3 | 15.3 |
| 3 | 26.7 |
