Figure 3.

mRNA-seq profiling of 7KCh-treated cardiac cells. (A) HL-1 cells were untreated (Con) or treated with 20 μM 7KCh for 24 h and harvested for mRNA-seq analysis by Novogene. The principal component analysis (PCA) plot of the mRNA-seq data from control (green) and 7KCh treatment (red) groups is shown. (B) The volcano plot highlights the upregulated (up, red) and downregulated (down, blue) genes in 7KCh-treated cells (versus Con cells). The fold change (${\log }_2\left(\frac{7\mathrm{KCh}}{\mathrm{Con}}\right)$) is indicative of ${\log }_2\left(\frac{\mathrm{Expression} \mathrm{units} \left(\mathrm{in} \mathrm{FPKM}\right) \mathrm{of} \mathrm{the} \mathrm{gene} \mathrm{in} 7\mathrm{KCh}-\mathrm{treated} \mathrm{cells}}{\mathrm{Expression} \mathrm{units} \left(\mathrm{in} \mathrm{FPKM}\right) \mathrm{of} \mathrm{the} \mathrm{gene} \mathrm{in} \mathrm{control} \left(\mathrm{Con}\right)\mathrm{cells}}\right)$ for the specified gene (n = 3, q value < 0.001). (C) Gene association network and module crosstalk network of all significantly altered genes are shown. The default network was generated by Network Analyst. The size of the nodes is based on their degree values, with big size for large degree values. The color of nodes is proportional to their betweenness centrality values.