Figure 1.

AngII-induced gene expression changes in VSMCs. (A) Differential expression (DE) analysis was performed between 2-hour AngII- and vehicle-treated samples. The microarray experiment was performed in triplicate. The results of DE analysis are shown as a volcano plot (x-axis: log2 fold change, y-axis: −log10 (FDR, false discovery rate), based on the Benjamini–Hochberg correction of p-values. Selected members of the DUSP family are color-coded and text-labeled. The dotted line shows the significance threshold (FDR < 0.05) (B) PROGENy pathway analysis of an AngII-induced gene expression signature. Pathway activity was calculated for 14 PROGENy pathways (x-axis) and normalized to z-scores (y-axis), based on a random permutation of gene labels.