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. 2021 Dec 23;57(Suppl 1):S11–S26. doi: 10.1134/S0003683821100124

Fig. 4.

Fig. 4.

HPLC (336 nm) analysis after incubation of 1,4-naphthoquinone and Glu, Cys or Ser with chassis cells. (a): i—S. cerevisiae INVSc1 + 1,4-naphthoquinone; ii—S. cerevisiae INVSc1 + 1,4-naphthoquinone + Glu; iii—1,4-naphthoquinone + Glu. (b): i—S. cerevisiae INVSc1 + 1,4-naphthoquinone; ii—S. cerevisiae INVSc1 + 1,4-naphthoquinone + Cys; iii—1,4-naphthoquinone + Cys. (c): i—S. cerevisiae INVSc1 + 1,4-naphthoquinone; ii—S. cerevisiae INVSc1 + 1,4-naphthoquinone + Ser; iii—1,4-naphthoquinone + Ser. (d): i—E. coli BL21(DE3) + 1,4-naphthoquinone; ii—E. coli BL21(DE3) + 1,4-naphthoquinone + Ser; iii—1,4-naphthoquinone + Ser. (e): i—E. coli BL21(DE3) + 1,4-naphthoquinone; ii—E. coli BL21(DE3) + 1,4-naphthoquinone + Lys; iii—1,4-naphthoquinone + Lys. Samples were analyzed using linear gradient of 10–90% (vol/vol) acetonitrile-water (containing 0.1% formic acid) for 30 min at a flow rate of 1 mL/min.