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. 2021 Dec 13;10(12):3521. doi: 10.3390/cells10123521

Figure 1.

Figure 1

In vitro generation of human his-tagged tau protein. (A) Scheme of the main steps of his-tagged 1N4R tau protein production using the pET-21a (+) expression vector in Escherichia coli BL21 and purification by Ni-NTA and SP Sepharose columns. (B) The absorbance of extracted tau protein with a peak at 280 nm and the absorbance of CBD with a peak at 207 nm by UV-visible spectroscopy. It indicates that there is no interference between CBD and tau protein absorbance. (C) The quality of purified tau protein using SDS-PAGE. Representative SDS-PAGE of purified tau protein (~59 kDa) at two different concentrations of 0.8 mg/mL and 0.4 mg/mL. The same trends were detected in the SDS-PAGE data of at least three independent samples (n = 3).