Figure 1.

Cyclophilin A impairs efferocytosis of apoptotic macrophages: The effect of Cyp A on the efferocytic capacity of macrophages was determined by in vitro efferocytosis assay. (a) (i & ii). In vitro efferocytosis assay using confocal microscopy and flow cytometry assay at 1.5 h and 3 h post-treatment with Cyp A. (b) (i & ii). Confocal images, quantitative analysis of confocal images, (c) (i & ii). flow cytometry dot plots and efferocytosis rate of phagocytozed cells in the presence of Cyp A. Efferocytosis assay revealed that Cyp A-treated macrophages had a higher percentage of free cell-tracker green-stained apoptotic HCASMCs. Red-colored cells represent healthy phagocytes, and dual-positive cells (both red and green) represent efficient phagocytosis. * p< 0.05, *** p< 0.001.