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. 2021 Dec 20;10(12):3598. doi: 10.3390/cells10123598

Figure 2.

Figure 2

Cyclophilin A reduces cholesterol efflux of macrophages. Cholesterol efflux was measured in an in vitro system using a cell-based cholesterol efflux assay kit after exposing macrophage cells preloaded with labelled cholesterol in the presence of Cyp A to a cholesterol acceptor, such as HDL or human serum. (a) (i and ii). Percentage cholesterol efflux was evaluated by in vivo IVIS Spectrum Imaging assay; (b) cholesterol efflux in cells was measured using a cell-based cholesterol efflux assay kit. Efflux from Cyp A-treated macrophages was reduced in 5 h (41%), compared to control cells (82%); (c) gene-expression analysis of ABCA1 mRNA by RT-PCR. (d) (i and ii). Confocal images indicate increased cellular-level expression of CD 47 in Cyp A-treated macrophages, similar to untreated macrophages; * p< 0.05 was considered significant. (e) (i and ii). The expression of CD 47 and in Cyp A macrophages treated by immunoblotting. * p< 0.05, ** p< 0.01, *** p< 0.001 for CD 47 and # p< 0.05, ## p< 0.01 ### p< 0.001 for Cyp A was considered significant. The data were compared using a one-way ANOVA, followed by multiple-comparison test using an SNK test.