Figure 3.

Cyclophilin A is associated with increased expression of CD 47 and is associated with reduced expression of the phagocyte receptor ligand calreticulin. The molecular association between Cyp A and known ligand receptors involved in efferocytosis was analyzed using molecular modeling studies. (a). Protein-protein docking analysis. CALR (PDB ID 1CWA) binds with Cyp A (PDB ID 3POW), with a high docking score of −219 and a ligand RMSD value of 51.69 Å. (b,c). Analysis of amino acids between two candidate proteins, CALR and Cyp A. (d) (i and ii). Confocal images indicate an increased cellular-level expression of calreticulin in Cyp A-treated macrophages. (e). Western blot images displaying increased CD 47 expression over cell-surface calreticulin in Cyp A-treated macrophages. * p < 0.05, ** p < 0.01was considered significant. The data were compared using a one-way ANOVA, followed by multiple-comparison test using an SNK test.