Table 2.
Differentially abundant proteins in ovine M. longissimus lumborum (LL) and M. psoas major (PM) muscles at day 1 or day 5 postmortem storage (4 °C).
| Spot. (a) | Protein Name (b) | Uniprot ID (b) | Gene Names | Protein Score | Mw/pI (c) | Matched Peptides (d) |
Sequence Coverage (%) (d) | Overabundant in Muscle (e) |
|---|---|---|---|---|---|---|---|---|
| Sarcoplasmicproteins (The early stage of storage, day 1, corresponding Figure 2A,B) | ||||||||
| 1314 | Serum albumin | P14639 | ALB | 153 | 71,139/5.80 | 15 | 43 | LD |
| 1404 | Creatine kinase M-type | W5PJ69 | CKM | 127 | 43,213/6.66 | 15 | 40 | LD |
| 1410 | Fructose-bisphosphate aldolase | W5PCA0 | ALDOB | 96 | 41,555/7.57 | 12 | 34 | LD |
| 1417 | Glyceraldehyde-3-phosphate dehydrogenase | W5PDG3 | GAPDH | 111 | 36,110/8.51 | 11 | 40 | LD |
| 1448 | Carbonic anhydrase 3 | W5PUC1 | CA3 | 83 | 29,726/7.70 | 6 | 22 | LD |
| 1469 | Heat shock protein family B | W5P9U1 | HSPB11 | 75 | 15,737/5.08 | 5 | 34 | LD |
| 1475 | Adenylate kinase isoenzyme 1 | C5IJA8 | AK1 | 172 | 21,750/8.40 | 15 | 62 | LD |
| Sarcoplasmicproteins (The late stage of storage, day 5, corresponding Figure 2C,D) | ||||||||
| 1058 | Pyruvate kinase | W5QC41 | PKM | 147 | 58,551/7.24 | 20 | 40 | LD |
| 1122 | Creatine kinase M-type | W5PJ69 | CKM | 109 | 43,213/6.66 | 14 | 37 | LD |
| 1128 | Fructose-bisphosphate aldolase | W5PCA0 | ALDOB | 87 | 41,555/7.57 | 12 | 36 | PM |
| 1134 | Glyceraldehyde-3-phosphate dehydrogenase | Q28554 | GAPDH | 95 | 36,110/8.51 | 13 | 39 | LD |
| 1172 | Adenylate kinase isoenzyme 1 | C5IJA8 | AK1 | 147 | 21,750/8.40 | 14 | 64 | LD |
| Myofibrillar proteins (The late stage of storage, day 5, corresponding Figure 2E,F) | ||||||||
| 2172 | Actin, alpha 1 | W5NYJ1 | ACTA1 | 70 | 42,338/5.23 | 11 | 25 | LD |
| 2242 | Enolase 2 | W5P5C0 | ENO2 | 117 | 47,382/7.60 | 14 | 37 | LD |
| 2323 | Fructose-bisphosphate aldolase | W5PCA0 | ALDOB | 62 | 41,555/7.57 | 7 | 26 | LD |
| 2327 | Creatine kinase M-type | W5PJ69 | CKM | 104 | 43,213/6.66 | 16 | 32 | LD |
| 2330 | Myosin light chain 1 | A0A0H3V7A0 | MYL1B | 73 | 20,950/4.95 | 9 | 58 | LD |
(a) The numbered spots in gel image (Figure 2). (b) Protein names and accession numbers were taken from the Uniprot database (http://www.uniprot.org (accessed on 8 October 2021)). (c) Theoretical protein mass (Mw; kDa) and isoelectric pH (pI). (d) Number of peptides that matched the protein sequence and total percentage of sequence coverage. (e) Muscle with greater abundance of the protein significance level was indicated (p < 0.05).