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. 2021 Dec 20;10(12):3154. doi: 10.3390/foods10123154

Figure 5.

Figure 5

GTE stimulates p53 but not p21 in MDA-MB-231 cells. Immunocytochemistry assay demonstrated p53 distribution on cells (A). p53 and p21 protein levels were accessed by Western blotting and GAPDH or β-actin were used as controls. The densitometric quantification of bands shows p53 (n = 5) (B,C) and p21 (n = 3) (D,E) levels in MDA-MB-231. Cell viability of GTE-treated cells was assessed by Trypan Blue in the presence of a p53 inhibitor (pifithrin-α) and results were expressed as % of controls (n = 3) (F). Symbol * indicates significant difference from controls (p < 0.05).