Table 2.
Digital droplet assays for copy number analysis of MSY multicopy genes and SRY.
| Gene | Forward Primer 5′–3′ | Reverse Primer 5′–3′ | Probe Sequence 5′–3′ |
|---|---|---|---|
| TSPY | CATAGTGGAGGAAGAGGATGAAA | GGCAATGGTTTAACCCTGAAA | CTCTTTCTGGGAGACCTGCCCTTT |
| SRY | TTCTGTGATCTATGCTGGCG | TTACCCTCCGGACTTTCTCA | AACAGGGACTCTGCCGCCACCA |
| RBMY | GAAGCTCCACAACTTGAGGT | CTCTGACCTATGATGGAAGCA | TGTCTGCCACCATGCTCACGACCA |
| ETSTY1 | GACGGACGACCTTGTGTT | ACGCTCACAGATGACAGTAG | TGTCCCGGCCACCTCAGGGC |
| ETSTY2 | TTGTTGTTAGGCTACCTGGC | AAGGGCAAACCATAACCTCC | TGGGCAAGCTTCTCCATGGTTGCTGCA |
| ETSTY5 | GAGGCAGGTACTTCGTTACC | TCACTCACAAAGTCAACGCT | TGCCGTGAGCTTGAGGGCGAA |
| UBA1Y | TTTCTGTTGTCTGGACGGAG | CTCCACGGATGTAGTCAGAG | AGCAGAGGCCTCCTGTGTCTGAGCT |
| HSFY | AGGCTTTCTCCACTGGTTTC | GAGGCTGTCCCGAACTTTTA | CCCCTGCTCTAAAGTGCTTCCTGTCG |
| MYOZ1 | GACTTTCCAGATGCCCAAGT | ACCAGAACCTCTCCAACAGGCCTTCT | GCTCCTCTGTTTCTCCATCC |