Table 2.

RECQL4 Protein interactions.

Process	Protein	Detection Methods *	Interaction Region	Function	References
Localization	p300	Co-IP, Co-Loc, Pull Down	1–408 aa	RECQL4 cellular localization	[9]
Replication	Cut5	Co-IP	N-terminus	DNA Replication, Xenopus Cut5 with xRTS	[48]
	MCM7	Co-IP, MS	ND	DNA Replication	[49]
	MCM10	Co-IP, MS, Pull Down	1–200 aa	DNA Replication, inhibition of RECQL4 helicase activity	[49]
	SLD5	Co-IP, MS	ND	DNA Replication	[49]
Telomere	TRF1	Co-Loc	ND	Telomere maintenance, Stimulates RECQL4 helicase activity	[50]
	TRF2	Co-IP	ND	Telomere maintenance, Stimulates RECQL4 helicase activity	[50]
	WRN	Co-IP	ND	RECQL4 stimulates WRN telomeric D-loop resolution	[50]
DSB Repair	BLM	Co-IP, Y2H	1–471 aa	Increase RECQL4 retention time at DSB sites, BLM stimulation	[51]
	KU70	Co-IP	N-terminus	NHEJ, RECQL4 enhances KU complex DNA binding. KU inhibits RECQL4 helicase activity	[52]
	KU80	Co-IP	ND	NHEJ, RECQL4 enhances KU complex DNA binding. KU inhibits RECQL4 helicase activity	[52]
	MRE11	Co-Loc, IP, Pull Down	N-terminus	HR, DNA end resection	[53]
	RAD51	Co-Loc, Co-IP	ND	DSB Repair	[54]
BER	APE1	Co-Loc	ND	APE1 endonuclease stimulation	[55]
	FEN1	Co-Loc	ND	FEN1 incision stimulation	[55]
	OGG1	Co-IP	N-terminus	Stimulates OGG1 AP lyase activity	[56]
	PARP1	Co-IP, PDS	833–1208 aa	Base excision repair	[57]
	POL β	ND	ND	Stimulates POLβ DNA synthesis activity	[55]
NER	XPA	Co-IP, Co-Loc, Fractionation, Pull Down	ND	Nucleotide excision repair	[58]
Mitochondria	p53	Co-IP, Co-Loc, Fractination	270–400 aa	Sequestering p53 from nucleus, mtDNA synthesis	[44]
	TOM20	Pull Down	13–18 aa	Mitochondrial import	[44]

* Abbreviations: Co-IP, Co-Immunoprecipitation; Co-Loc, Co-Localization; IP, immunoprecipitation; MS, mass spectrometry; ND, not determined; PDS, phage display.