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RT-qPCR shows a significant increase in the expression of plasmid-borne mutant sup35-218 and sup45-105 alleles. Shown are relative expression levels of the corresponding genes estimated using the CT method (see Materials and Methods). ADH1 was used as the reference gene in all cases. n/s — p > 0.05; *** — p < 0.001 in the Wilcoxon–Mann–Whitney test.
