Table 1.
Validation data for the optimized analytical method using HILIC-CAD, including concentration range, linear equation, correlation coefficients, LOD, LOQ, intra- and inter-day precision, and accuracy.
| Saccharides | Fructose | Glucose | Sucrose | 1-Kestose | Nystose | Fructofuranosyl Nystose |
|
|---|---|---|---|---|---|---|---|
| Concentration range (µg/mL) |
0.5–100 | 0.5−100 | 0.5−100 | 0.5−100 | 0.5−100 | 0.5−100 | |
| Linear equation | y = −0.0003x2 + 0.1193x + 0.0279 | y = −0.0003x2 + 0.1268x + 0.0577 | y = −0.0003x2 + 0.1237x + 0.0781 | y = −0.0003x2 + 0.1149x + 0.0539 | y = −0.0002x2 + 0.0980x + 0.0329 | y = −0.0002x2 + 0.0995x + 0.0241 | |
| R2 | 0.9998 | 0.9998 | 0.9996 | 0.9998 | 0.9997 | 0.9999 | |
| LOD/LOQ (µg/mL) |
0.25/0.5 | 0.25/0.5 | 0.25/0.5 | 0.25/0.5 | 0.25/0.5 | 0.25/0.5 | |
| Precision (%) | Intra-day (n = 9) |
0.733 | 0.768 | 0.639 | 0.668 | 0.848 | 0.840 |
| Inter-day (n = 3) |
0.630 | 1.46 | 1.02 | 1.67 | 1.46 | 1.71 | |
| Accuracy (%) |
96.5 | 91.7 | 94.3 | 92.3 | 98.2 | 97.9 | |