Fig. 2.

Assay results with a benchtop fluorescence microscope. Pixel counts from three different images of a single channel were summed and used as a single data point. Experiments were repeated four times (each time with three images), each using a different paper microfluidic chip. Average pixel counts (n = 4) are plotted with the error bars representing standard errors, each time with different spraying/capture and with different paper microfluidic chips. (a) With two-times spraying, and (b) with five-times spraying. * denotes p ≤ 0.05, **p ≤ 0.01, and n.s. = not significant.