Figure 3.

Effect of lysis solution. When choosing a lysis solution, FSC and SSC properties should be closely monitored to detect the introduction of artifacts. Lysis solutions can decrease the cell size and therefore decrease the FSC resulting in a poor distinction between red blood cells, lymphocytes, and WBC. (A and B) The distinction between WBC (blue), lymphocytes (green), and red/dead cells (red) are clearly seen with ammonium chloride (NH₄Cl) as lysis buffer. (C and D) The same sample is lysed with FACS Lyse sample, but other settings remained the same, resulting in less difference between cell populations. FACS = fluorescence-activated cell sorting; FSC = forward scatter; SSC = side scatter; WBC = white blood cells.