Figure 7.

HOVON-P1 and ELN tube comparison. (A) Comparison of log-transformed blast percentage of 62 samples (both diagnose and follow-up) between HOVON-P1 tube (the first tube used from the Heamato Oncology Foundation for Adults in the Netherlands) (x-axis) and the ELN tube (y-axis) with a correlation coefficient of r = 0.99 (P < 0.001). (B) LAIP percentage compared on the same 62 samples as panel A (r = 0.98, P < 0.001). (C) Measurable residual disease (MRD) percentage (y-axis; split into 2 segments on MRD 0.25%) of all 12 follow-up samples per center. A total of 6 follow-up samples are measured with 2 different tubes (HOVON P1 and ELN tube). The centers and patient data are anonymized. The associated samples are next to each other on the x-axis with corresponding numbers. Samples used are at different time points during therapy and with different cytogenetic alterations. Three (HOVON sample 1 and 6; ELN sample 4) of the 12 samples did not have an anonymous MRD result between the 4 centers if the 0.1% cutoff would be used, although not all samples were collected after induction therapy. These differences could be explained when LAIP gates were individually examined. Other 9 samples had identical MRD result (4 MRD+ and 5 MRD–). ELN = The European LeukemiaNet; LAIPs = leukemia-associated immunophenotypes; MRD = measurable residual disease.