Table 1.
Formula for Detecting Hemodilution | Additional Requirements |
---|---|
Bone marrow purity = [1-(erythrocytes BM/erythrocytes PB) × (leukocytes PB/leukocytes BM)] × 100% | Matched PB16 |
PB contamination index = −3.052 + 0.065 × (%CD10+ neutrophils of granulocytes) −0.609 × (%CD34+)−2.008 × (%plasma cells) | CD10, CD34 positive cells and plasma cells12 |
Normalized blast count = (80%/% dim CD16) × blast count | CD1613 |
Predicted bone marrow purity = [1 – (Lymphocytes FCM / Lymphocytes PB) × (Leukocytes PB / Leukocytes FCM)] × 100% | Matched PB14 |
Suggested blood contamination if mast cell population (CD117+) ≤ 0.002% | CD117 positive mast cells15 |
BM = bone marrow; FCM = flow cytometry; PB = peripheral blood.