Skip to main content
. 2021 Dec 22;6(1):e676. doi: 10.1097/HS9.0000000000000676

Table 1.

Overview of Formulas Proposed for Calculating Hemodilution

Formula for Detecting Hemodilution Additional Requirements
Bone marrow purity = [1-(erythrocytes BM/erythrocytes PB) × (leukocytes PB/leukocytes BM)] × 100% Matched PB16
PB contamination index = −3.052 + 0.065 × (%CD10+ neutrophils of granulocytes) −0.609 × (%CD34+)−2.008 × (%plasma cells) CD10, CD34 positive cells and plasma cells12
Normalized blast count = (80%/% dim CD16) × blast count CD1613
Predicted bone marrow purity = [1 – (Lymphocytes FCM / Lymphocytes PB) × (Leukocytes PB / Leukocytes FCM)] × 100% Matched PB14
Suggested blood contamination if mast cell population (CD117+) ≤ 0.002% CD117 positive mast cells15

BM = bone marrow; FCM = flow cytometry; PB = peripheral blood.