Figure 5.

Inhibition of Histone acetylation in articular chondrocytes results in the increased expression of TNF-α promoter. Figure 5: Normal and OA cartilage was treated with anacardic acid (4μM). ChIP was performed to measure acetylated H3K9/K14 (A), acetylated H4K12 (B), HAT1 (C) and CBP (D) occupancy in the region of the TNF-α promoter in normal and OA cartilage treated with anacardic acid. The results were normalized to the percentage of the input control. Total protein was isolated for western blot analysis using TNF-α antibody (E) β-actin was used as an internal control. Total RNA was isolated from normal and OA cartilage with and without anacardic acid treatment for quantitative RT-PCR using TNF-α primers (F). Data are shown as the means ± SD. ∗P < .05, ∗P < .05, anacardic vs vehicle. The data were obtained from 37 OA patients receiving knee replacement surgery and 13 samples of non-arthritic tissues obtained during amputation.