TABLE 1.
Origins and Tm analysis of strains used in this studya
| Species and sample (n) | Location (yr) of isolation | Mean Tm ± SD (°C) |
|---|---|---|
| B. burgdorferi sensu stricto ATCC 35210 (1) | United States | 84.02 |
| B. garinii | ||
| 387 CSF (1) | Germany | 81.62 |
| Human samples (8) | Finland (1996–1999) | 80.62 ± 0.33b |
| Tick samples (10) | Finland (1996–1998) | 81.30 ± 0.40 |
| B. afzelii | ||
| Bo 23 (1) | Germany | 83.71 |
| Human samples (1) | Finland (1999) | 83.84 |
| Tick samples (10) | Finland (1998–1999) | 83.42 ± 0.27 |
a
DNA was extracted from the Borrelia reference strain, human skin biopsy samples, and tick midguts and was subjected to real-time PCR for the recA gene; melting curve analysis was also performed.
b
P = 0.0014 when compared to Tm of recA PCR products amplified from tick samples.