Figure 2.

Morphology of human stroke brain tissue. Morphological evaluation of the temporal lobe tissue, HE staining. (A,B), Control non-stroke samples; (C,D), Brain tissue collected at 48 h after stroke onset. Black arrows: eosinophilic (red) neurons with darkly stained pyknotic nuclei. Blue arrows: necrotic “ghost” neurons with an absent nuclear detail. Stars: peri-neuronal and peri-vascular space widening associated with edema. Neutrophils (arrowheads) were detected in the vicinity of large vessels and capillaries (E,F), tissue collected at 60 h after stroke onset), as well as in the brain parenchyma (G,H), tissue collected at 28 h after stroke onset). (E) and higher magnification in (F): Margination and pavementing of neutrophils along the blood vessel wall; some neutrophils migrated out and are detected in the blood vessel vicinity. (G) and higher magnification in (H): Neutrophils are detected at a considerable distance from the blood vessels, which demonstrates the process of neutrophil invasion into the damaged brain tissue. BV- blood vessel. Bars: (A,C): 50 μm; (B,D,E–H): 20 μm.