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. 2021 Dec 16;2021:3097432. doi: 10.1155/2021/3097432

Figure 2.

Figure 2

Strategy for generating the ADAM8 gene knockdown mice. (a) Effect of stereotactic cerebral injection, n = 4 mice per group. (b) Immunofluorescence analysis of adeno-associated virus in primary motor cortex region of mice 28 days after injection. Adeno-associated viruses carry green fluorescent protein (EGFP) and DAPI (blue) stains the nucleus (scale = 200 μm). The magnification is 10x, n = 3 mice per group. (c) After reperfusion for 24 hours, the effects of different shRNA fragments were analyzed with western blotting, n = 5 mice per group. (d) The expression of ADAM8 in the cortex of mice in different treatment groups was detected by immunofluorescence staining, the magnification is 40x, and the scale bar was 50 μm, n = 5 mice per group. All values are expressed as the mean ± SD. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 vs. Sham Scramble shRNA group. #P < 0.05, ##P < 0.01, ###P < 0.001 vs. MCAO Scramble shRNA group.