Figure 4.

ADAM8 promoted the apoptosis of cerebral cortical neurons in cerebral ischemia and reperfusion mice. (a) Cleaved caspase-3 in the penumbra was detected by western blotting after reperfusion for 24 hours, n = 4 mice per group. (b) TUNEL and immunofluorescence analysis of the apoptosis cerebral in cortex penumbra at 24 hours after I-R injury. The magnification was 40x and the scale bar was 50 μm, n = 3 mice per group. All values are expressed as the mean ± SD. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001 vs. Sham Scramble shRNA group. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. MCAO Scramble shRNA group.