Skip to main content
. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: J Immunol. 2021 Nov 24;208(1):16–26. doi: 10.4049/jimmunol.2100643

Figure 2. The DR1-CAR expressed by transfected cells is recognized by CD4+ T cells in an MHC restricted, antigen specific manner.

Figure 2.

The chimeric DR1 molecules were transfected into 293T cells and tested for their ability to be recognized as antigen specific ligands using T cell hybridomas specific for CII or HA peptide. The DR1-CII chimeric molecule was recognized by the CII-specific CD4+ T cells (■ bars) as indicated by the production of IL-2, but not by the HA-specific T cells. CAR T-cells expressing empty DR1 (DR1-CAR) did not stimulate the CII-specific T cells unless the CII peptide was added exogenously to the cultures (■ bars). In both instances, the positive response of the two CAR T-cells were similar to that observed using spleen cells as APC from B6.DR1 mice (☐ bars). These data indicate that the chimeric DR1-CII-CD28-CD3 CAR molecule functions as an MHC restriction element. Experiment was repeated at least 3 times; error bars indicate standard error of the mean (SEM); ★ indicates p value < 0.0001.