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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: J Immunol. 2021 Nov 24;208(1):16–26. doi: 10.4049/jimmunol.2100643

Figure 8. Phenotype of DR1 CAR T cells maintained in culture.

Figure 8.

Expression of exhaustion markers PD1, CD69, and Tim-3 (panel A) and CTL memory markers (panel B) were measured from day 10 post transduction through day 65 in culture. For in vitro maintenance, the CAR T cells were seeded at 1 × 106 cells/ml in complete RPMI 1640 with 20 U/ml of IL-2 and were re-stimulated weekly by culture in tissue culture plates coated with anti-CD3 and anti-CD28 antibody (1 ug/ml). Cells were labeled with fluorochrome-conjugated antibodies specific for CD3, CD4, CD8, DR1, Tim-3, PD1, CD25, CD69 (panel A), or CD3, CD4, CD8, DR1, CD62L, and CD44 (panel B). Data shown were gated on CD3+, CD4−, CD8+, and DR+ and are based on a minimum of 10,000 cells analyzed. Data are representative of 6 different CAR T cell lines.