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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: J Immunol. 2021 Nov 24;208(1):16–26. doi: 10.4049/jimmunol.2100643

Figure 9. CTL activity of DR1-CII CAR T cells maintained in culture.

Figure 9.

CTL activity of the CAR T cells was measured using a flow cytometry based assay. CAR T cells and target cells (CII-specific T cell hybridoma) were mixed at a 2:1 effector to target ratio, and CTL lysis measured at 4 and 24 hrs. Despite the expression of exhaustion markers, DR1-CII CAR T cells were active as CTL in the lysis of CD4+ T cells to similar levels from day 20 through day 62 in culture. To measure lysis, cells were labeled with fluorochrome conjugated antibodies specific for TCR-BV8.3 (marker for CII-specific T cell targets), CD8, and DR, and data are based on gates with a minimum of 10,000 cells analyzed. Data are representative of a minimum of 3 different CAR T cell lines.