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. 2021 Dec 10;12:756068. doi: 10.3389/fpls.2021.756068

FIGURE 5.

FIGURE 5

Phenotypic analysis of group IV CaSAP genes to the dehydration stress. (A) Dehydration-tolerant phenotypes of plants transformed with group IV TRV2:CaSAP. Four-week-old plants of each line were subjected to the dehydration stress by withholding watering for 14 days followed re-watering for 3 days. The percentages of surviving plants were calculated after re-watering. (B,C) Effect of dehydration stress on electrolyte leakage (B) and lipid peroxidation (C) in the leaves of TRV2:00 and group IV TRV2:CaSAP pepper plants. Four-week-old plants were subjected to the dehydration stress by detaching shoot for 6 h (n = 20 plants of each line per replicate, 3 replicates). (D) Expression analysis of dehydration-inducible genes in the leaves of TRV2:00 and group IV TRV2:CaSAP pepper plants. The relative expression (ΔΔCT) of CaSAP was normalized to that of CaACT1, which was used as an internal control gene. All values are presented as the mean ± standard error of values from three independent experiments. Different letters indicate significant differences among control and TRV2:CaSAP pepper plants (ANOVA; P < 0.05).