FIG. 8.
Expression of the spin gene during development. (A) In situ hybridization of the spin mRNA probe to whole-mount wild-type embryos at stage 12 is shown in the first set of images. A dorsal view (top) and a dorsolateral view (middle) of an embryo are shown. The boxed region is illustrated at a higher magnification in the bottom image. Expression is evident in the developing VNC and brain. The three middle panels illustrate mRNA localization in the CNS of a third-instar larva (3L) (left), pupa (middle), and adult (right) of the CS wild type. The eye-antennal disks attached to the third-instar brain can also be seen. Expression in the larval ring gland is shown in the inset. The far-right images show mRNA expression in the spinP1 adult. The four CNS images show the dorsal side up. (B) The top three images show the double staining of the spinP2/CyO larval brains for the spin reporter β-Gal (green) and the Repo protein (red). Colocalization of β-Gal and the Repo protein results in a yellow signal. The bottom three images show the double staining of the spinP2/CyO pupal brains for spin reporter β-Gal (green) and the Elav protein (red). The spin gene is not expressed in the neurons. α, antibody. (C) The left two images show β-Gal expression in spinP2/CyO ovaries. The right two images show the in situ hybridization analysis of spin gene expression in ovaries at stage 10A (st10A) and st12. The spin gene is expressed in the follicle cells at st10B and st12.