Figure 1.

Vectorization of the Wildtype AAV Genome to Produce Recombinant AAV

(A) The 4.8kb wildtype adeno-associated virus (AAV) genome is comprised of replication (rep) and capsid (cap) genes flanked by inverted terminal repeats (ITRs). (B) Vectorization of AAV requires the expression construct containing ITRs in cis flanking the promoter, transgene, pA (polyadenylation signal) and optionally the WPRE (post-transcriptional regulatory element). Also required is a packaging plasmid and adenoviral helper plasmid which provide the required elements in trans for vector packaging.