Figure 3.

Screening AAV Variants for Entry and Gene Expression Into Cardiomyocytes

(A) Generation of the shuffled adeno-associated virus (AAV) capsid library using the functional transduction (FT) platform. The construct contains both the shuffled cap gene and green fluorescent protein (GFP) under the control of a cardiac specific promoter (CSP). (B) Transduction of cells, followed by sorting to isolate GFP-positive cells. DNA is then extracted and the cap gene recovered. The cap gene is then recloned back into the library and taken for further rounds of sequencing. (C) Enriched AAV capsid candidates at the end of successive rounds of selection. hiPSC-CM = human-induced pluripotent stem cell derived cardiomyocyte; ITR = inverted terminal repeat; PCR = polymerase chain reaction