Figure 5.

Profiling of immune-associated genes in 4T1 tumors in mice primed with miPSCs. Transcriptome analysis was performed on tumors from untreated mice and from mice primed with 6 doses of miPSCs and VPA in order to find genes that were differentially expressed between the two conditions. Total RNA was extracted from 4T1 tumors in treated and control mice, following the instructions of the manufacturer (TRIzol, Life Technologies). Microarray probes was synthetized by one cycle of RNA amplification in which molecules were labeled in an Affymetrix microarray station (Affymetrix, CA). Labeled microarray probes were hybridized on a Mouse Clariom S (mm10) microarray (Thermo Fisher Scientific). (A) Differentially expressed genes (DEGs) between control and vaccinated mice xeno-transplanted with 4T1 cancer cells; expression heatmap was produced with an unsupervised classification algorithm (Euclidean distances, Ward method). (B) Barplot of functional enrichment in Gene Ontology Biological Processes of genes that were overexpressed in vaccinated mice. (C) Immune profiling carried out by gene-set enrichment analysis on the transcriptome of 4T1-transplanted mice (NES: normalized enriched score). (D) Genes associated with the immune network that were enriched in 4T1 tumors from miPSC+VPA-treated mice.