Fig. 2.

Panobinostat alone and combined with olaparib reduces HR proficiency in ID8-luc cells. HR repair efficiency of DNA double-strand breaks in drug-treated ID8-luc cells was measured in three assays. Cells were pre-treated with 0.5µM cisplatin for 6 h were then treated with panobinostat (25 nM), olaparib (10 μM) or the combination for a further 24 h, and formation of BRCA1 (A&B) and RAD51 foci (C&D) analyzed by IF. Representative BRCA1 and RAD51 images (green) are shown; DAPI-stained nuclei are in blue. (E&F) IF analysis of GFP expression in ID8-luc cells co-transfected with the pDRGFP and I-Sce1 plasmids (both 1 µg). Cells were then treated with panobinostat (25 nM), olaparib (10 μM) or the combination for 24 h. At least 100 cells were counted (x40) for each treatment in the three assays. Values are mean+SEM; *p < 0.01 compared to control; ^ap < 0.01 relative to olaparib alone, Student's t test (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.).