Fig. 6.

Panobinostat reduces expression of tumor epithelial and M2 macrophage markers and increases expression of pan-macrophage and M1 markers in ascites​ cells from ID8-luc-injected mice. C57BL/6 mice were injected with ID8-luc cells. 30 days after injection, mice were treated with vehicle, panobinostat (2.5 mg/kg 5 times weekly PO), olaparib (100 mg/kg 5 times weekly PO) or panobinostat + olaparib combination for 4 weeks. After sacrifice, ascites was harvested and mRNA extracted from the cell pellet. Drug effects on steady state mRNA expression of (A) epithelial marker CK18, (B) macrophage marker F4/80, (C) T cell recruitment factor CXCL9, (D) angiogenic factor VEGFA, (E, F) M1 macrophage markers CCL3 and iNOS) and (G, H) M2 macrophage markers, arginase-1 and mannose receptor were measured by qRT-PCR. Relative expression values are expressed as mean±SEM, calculated using the 2^−ΔΔCt method relative to corresponding GAPDH levels. *p < 0.01 single drug effect relative to control; ^ap < 0.01 relative to olaparib alone; ^bp < 0.01 relative to panobinostat alone, Mann-Whitney test.