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. 2021 Dec 9;20:148–164. doi: 10.1016/j.csbj.2021.12.009

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© 2021 Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 8 — Digestion of xylan and arabinoxylan substrates by differentially expressed CAZymes from barley straw and total tract indigestible residue rumen cultures. (A) Substrates used to detect GH11 activity: oat spelt and beechwood heteroxylan, and rye and wheat arabinoxylan. (B) Thin layer chromatography of GH11 substrates without (-) and with (+) GH11 enzymes, as indicated. (C) Soluble products of enzymatic digestion were analyzed by HPAEC-PAD using a gradient of 10–120 mM sodium acetate in a constant background of 30 mM NaOH. Xylan mono- and oligosaccharide standards were included as controls: xylose (X), xylobiose (X₂), xylotriose (X₃), xylotetraose (X₄), xylopentaose (X₅), and xylohexaose (X₆), and/or a xylose ladder of X₁-X₆ forms. Arabinoxylan oligosaccharide standards are as follows: 3²-α-L-arabinofuranosyl-xylobiose (A³X), 2³-α-L-arabinofuranosyl-xylobiose (A²XX), 2³,3³-di-α-L-arabinofuranosyl-xylotriose (A^2,3XX).