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. 2021 Dec 10;12:780588. doi: 10.3389/fphar.2021.780588

FIGURE 3.

FIGURE 3

PRE-084 and BD1063 reduce spinal MN degeneration and decrease microgliosis in SOD1G93A female mice at 16 weeks of age (A) Quantification of surviving MNs (mean number of MNs per section ±SEM) in the lumbar spinal cord, showing improved MN preservation with Sig-1R ligands PRE-084 and BD1063. (B) Representative spinal cord images of MNs stained with cresyl violet. Scale bar 100 μm. (C, E) Representative images of immunoreactivity for Iba1 (C), a marker for microglia, and GFAP (E), a marker of astrocytes, in the ventral horn of WT and SOD1G93A mice with or without Sig-1R treatments. Scale bar 50 μm. D, F) Graphs showing the quantification of percentage of Iba-1 (D) and GFAP (F) immunolabeling in ventral horn of spinal cord (n = 10 WT, n = 10 SOD1 saline, n = 8 SOD1 BD1063, n = 7 SOD1 SA4503 0.25 mg/kg, n = 7 SOD1 SA4503 1 mg/kg and n = 10 SOD1 PRE-084 mice). *p < 0.05 vs SOD1G93A saline mice.