Nested PCR products amplified from representative tick samples. Lane M, DNA marker. Sizes (in base pairs) are indicated on the left. Lane 1, positive control (plasmid containing the E. chaffeensis 16S rRNA gene); lane 2, A. testudinarium adult from cattle; lane 3, H. yeni adult from M. reevesi; lane 4, H. yeni adult from cattle; lane 5, H. yeni adult from Caprologus sinensis; lane 6, H. yeni nymph from L. sinensis; lane 7, water (as a negative control). The expected product size is 389 bp.