FIGURE 5.

Probiotic lyophilized formulations can modulate gene expression of epithelial barrier genes in the ileum section of mice with 5-FU-induced mucositis. Gene for zonula occludes 1 and 2 (zo-1 and zo-2, respectively), occludin (ocln), and claudin-1 (cln-1) were measured by RT-qPCR. The animals were gavage with: PBS pH 7.4 (Naive group and 5-FU inflamed control group); 12% skimmed milk supplemented with 30% WPI and Fructooligosaccharide (Matrix); a formulation containing L. casei BL23, a formulation containing the mix of probiotics (Symbiotic). Means and standard deviations are calculated from 3 animals per group, from 2 independent repetitions and each quantification was performed in duplicate (technical duplicate). Asterisks represent statistically significant differences between strains and were indicated as follows: *p < 0.05; **p < 0.01; ***p < 0.001 and ****p < 0.0001.