Table 4.
Primary and Secondary Antibodies
| Antigen detected by primary antibody | Host | Dilution | Source | Research Resource Identifier (RRID) | |
|---|---|---|---|---|---|
| Calbindin D-28 | Rabbit | 1:400 | Swant (CB38A); Berne, Switzerland | RRID:AB_10000340 | |
| Calretinin | Rabbit | 1:400 | Swant (7697) | RRID:AB_2721226 | |
| Calretinin | Goat | 1:400 | Swant (CG1) | RRID:AB_10000342 | |
| CD31 | Rat | 1:300 | BD Pharmingen (557355); San Diego, CA | RRID:AB_396660 | |
| CGRP | Mouse | 1:200 | Abcam (ab81887); Boston, MA | RRID:AB_1658411 | |
| αCGRP | Rabbit | 1:500 | Peninsula Labs (T4032), San Carlos, CA | RRID:AB_518147 | |
| ChAT | Goat | 1:100 | Millipore (AB 144P), Burlington, MA | RRID:AB_262156 | |
| Enkephalin | Mouse | 1:200 | Millipore (MAB350) | RRID:AB_2088149 | |
| Enkephalin | Mouse | 1:150 | Abcam (ab150346) | Not Found | |
| GABA | Rabbit | 1:300 | Sigma (A2052), St. Louis, MO | RRID:AB_477652 | |
| Green Fluorescent Protein | Chicken | 1:300 | AVES (GFP1020), Davis, CA | RRID:AB_10000240 | |
| Green Fluorescent Protein | Goat | 1:400 | Abcam (ab5450) | RRID:AB_304897 | |
| Green Fluorescent Protien | Rabbit | 1:200 | Abcam (ab6556) | RRID:AB_305564 | |
| HuC/D (ANNA-1) | Human | 1:20,000 | Gift from Dr V. A. Lennon | RRID:AB_2314657 | |
| Iba1 | Rabbit | 1:500 | Wako Chemicals (019-19741), Richmond, VA | RRID:AB_839504 | |
| Neurofilaments | Mouse | 1:500 | ThermoFisher (13-1300), Waltham, MA | RRID:AB_2532999 | |
| neuronal nitric oxide synthase | Rabbit | 1:400 | Millipore (AB5380) | RRID:AB_91824 | |
| neuronal nitric oxide synthase | Rabbit | 1:300 | Abcam (ab76067) | RRID:AB_2152469 | |
| neuronal nitric oxid synthase | Goat | 1:200 | Invitrogen (OSN00004G), Waltham, MA | RRID:AB_10709561 | |
| PGP9.5 | Guinea pig | 1:500 | Neuromics (GP14104), Edina, MN | RRID:AB_2210625 | |
| red fluorescent protein | Chicken | 1:600 | Rockland (600-901-379), Philadelphia, PA | RRID:AB_10704808 | |
| red fluorescent protien | Rabbit | 1:600 | Rockland (600-401-379) | RRID:AB_2209751 | |
| S-100 | Rabbit | 1:350 | Abcam (ab52642) | RRID:AB_882426 | |
| Serotonin (5-HT) | Rabbit | 1:1000 | Immunostar 20080, Hudson, WI | RRID:AB_572263 | |
| Serotonin (5-HT) | Goat | 1:1000 | Immunostar 20079 | RRID:AB_572262 | |
| Somatostatin | Rat | 1:200 | Abcam (ab30788) | RRID:AB_778010 | |
| Somatostatin | Rat | 1:200 | Millipore (MAB354) | RRID:AB_2255365 | |
| Somatostatin | Rabbit | 1:200 | Abcam (ab108456) | RRID:AB_11158517 | |
| Substance P | Rabbit | 1:200 | Abcam (ab67006) | RRID:AB_1143173 | |
| Substance P | Rat | 1:150 | Abcam (ab7340) | RRID:AB_305866 | |
| Substance P | Guinea pig | 1:200 | Abcam (ab06291) | No longer available | |
| Synaptophysin | Rabbit | 1:150 | Abcam (ab32127) | RRID:AB_2286949 | |
| Synaptotagmin 1 | Rabbit | 1:100 | Abcam (ab131551) | RRID:AB_11157546 | |
| Synaptotagmin 1 | Chicken | 1:100 | Abcam (ab133856) | RRID:AB_2885088 | |
| TUJ1 (β III tubulin) | Rabbit | 1:1000 | Covance (AB 291637) discontinued; now BioLegend (PRB-435P-100), Saan Diego, CA | RRID:AB_291637 | |
| TUJ1 (β III tubulin) | Chicken | 1:500 | Abcam (ab107216) discontinued | RRID:AB_10899689 | |
| VIP | Rabbit | 1:300 | Immunostar (20077) | RRID:AB_572270 | |
| VIP | Rabbit | 1:100 | Abcam (ab22736) (used in initial experiments, new lots do not work) | RRID:AB_447294 | |
| VIP | Rabbit | 1:500 | Sigma (MPA017324) | Not found | |
| VIP | Guinea pig | 1:300 | Invitrogen (PA1-36030) | RRID:AB_1088264 | |
| Secondary antigen | Host | Conjugate | Dilution | Source | RRID |
| Chicken (IgY, H+L) | Goat | Alexa Fluor 488 | 1:300 | Invitrogen (A11039) | RRID:AB_142924 |
| Chicken (IgY, H+L) | Goat | Alexa Fluor 568 | 1:500 | Invitrogen (A11041) | RRID:AB_2534098 |
| Chicken (IgY, H+L) | Goat | Alexa Fluor 594 | 1:500 | Invitrogen (A11042) | RRID:AB_2534099 |
| Goat (IgG, H+L) | Donkey | Alexa Fluor 488 | 1:500 | Invitrogen (A11055) | RRID:AB_2534099 |
| Goat (IgG, H+L) | Donkey | Alexa Fluor 555 | 1:500 | Invitrogen (A21423) | Not Found |
| Goat (IgG, H+L) | Donkey | Alexa Fluor 647 | 1:500 | Invitrogen (A21447) | RRID:AB_141844 |
| Guinea pig (IgG, H+L) | Goat | Alexa Fluor 555 | 1:500 | ThermoFisher A-21435 | RRID:AB_2535856 |
| Guinea pig (IgG, H+L) | Goat | Alexa Fluor 594 | 1:500 | Invitrogen (A11076) | RRID:AB_141930 |
| Human (IgG, H+L) | Goat | Alexa Fluor 555 | 1:500 | Invitrogen (A21433) | RRID:AB_2535854 |
| Human (IgG, H+L) | Goat | Alexa Fluor 647 | 1:500 | Invitrogen (A 21445) | RRID:AB_2535862 |
| Human (IgG, H+L) | Goat | DyLight 405 | 1:500 | Novus Biologicals (NBP1-72871) lLittleton, CO | RRID:AB_11008096 |
| Human (IgG, H+L) | Alpaca | DyLight 405 | 1:100 | Jackson IR (609-475-213), West Grove, PA | RRID:AB_2721858 |
| Mouse (IgG, H+L) | Goat | Alexa Fluor 594 | 1:500 | Invitrogen (A11005) | RRID:AB_141372 |
| Rabbit (IgG, H+L) | Donkey | Alexa Fluor 488 | 1:500 | Invitrogen (A21206) | RRID:AB_2535792 |
| Rabbit (IgG, H+L) | Donkey | Alexa Fluor 555 | 1:500 | Invitrogen (A31572) | RRID:AB_162543 |
| Rabbit (IgG, H+L) | Donkey | Alexa Fluor 647 | 1:500 | Invitrogen (A31573) | RRID:AB_2536183 |
| Rabbit (IgG, H+L) | Goat | Alexa Fluor 488 | 1:500 | Invitrogen (A11034) | RRID:AB_2576217 |
| Rabbit (IgG, H+L) | Goat | Alexa Fluor 546 | 1:500 | Invitrogen (A11010) | RRID:AB_2534077 |
| Rabbit (IgG, H+L) | Goat | Alexa Fluor 568 | 1:500 | Invitrogen (A11011) | RRID:AB_143157 |
| Rabbit (IgG, H+L) | Goat | Alexa Fluor 594 | 1:500 | Invitrogen (A21207) | RRID:AB_141637 |
| Rabbit (IgG, H+L) | Goat | Alexa Fluor 647 | 1:500 | Invitrogen (A21245) | RRID:AB_2535813 |
| Rat (IgG, H+L) | Donkey | Alexa Fluor 594 | 1:400 | Invitrogen (A21209) | RRID:AB_2535795 |
| Rat (IgG, H+L) | Goat | Alexa Fluor 647 | 1:500 | Invitrogen (A21247) | RRID:AB_141778 |
| Sheep (IgG, H+L) | Donkey | Alexa Fluor 488 | 1:300 | Invitrogen (A11015) | RRID:AB_141362 |
| Sheep (IgG, H+L) | Donkey | Alexa Fluor 594 | 1:500 | Invitrogen (A11016) | RRID:AB_253408 |
| Sheep (IgG, H+L) | Donkey | Alexa Fluor 647 | 1:500 | Invitrogen (A21488) | Not found |
NOTE. All primary and secondary antibodies used in the reported studies are listed. The list includes the antigen recognized, the host that the antibody was raised in, the dilution used, the source, and RRID when available.
RRID, Research Resource Identifier; H+L, high and low.