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. 2021 Nov 29;13(12):2390. doi: 10.3390/v13122390

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Reverse-transcription PCR analysis of CRE-REP assay samples. Example gel electrophoresis analysis of total RNA isolated and amplified by RT-PCR from CRE-REP assays. In this example, we show results from both 33 °C (A) and 37 °C (B) following co-transfection of the EV68/CRE acceptor template with either a species B, C, or D donor template. Lanes are numbered 1–8 for reference. Numbers in brackets below gel images are the expected sizes for a precise recombinant of each assay template pairing.