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. 2021 Nov 29;13(12):2390. doi: 10.3390/v13122390

Figure 6.

Figure 6

Replication of inter-species recombinant EV68/PV1-1: (A) schematic of inter-species recombinant genome EV68/PV1-1. Open arrowheads mark positions of primers for cDNA synthesis and closed arrowheads mark positions of primers for PCR; (B) PCR assay for negative- and positive-strand RNA. Left-hand panels denote amplification of recombination window with primers specific to the recombinant (EV68(F) and PV1(R)), and right-hand panels denote amplification of EV-D68 controls with EV-D68 specific primers (EV68(F) and EV68(R)). Amplification from positive-strand (upper panels) and negative-strand (lower panels) RNA is shown. Arrows indicate expected product sizes.