Table 1.

IC₅₀ in human and murine sEH and microsomal stability values of 1, 6a–h and 10a–e.

Cmpd #	Human sEH	Murine sEH	Microsomal Stability 1
			Human	Mouse
	IC50 (nM) 2		% Remaining at 1 h
1	3.1	6.0	1.0	1.0
6a	34.5	0.4	0.3	0.0
6b	30.2	0.4	0.0	0.0
6c	1.6	0.4	5.5	5.1
6d	1.0	0.4	7.0	1.4
6e	2.8	0.5	0.6	0.2
6f	2.4	0.4	1.3	0.5
6g	37.5	3.3	0.1	0.1
6h	21.4	12.9	0.3	0.1
10a	0.6	0.7	0.1	0.1
10b	3.4	0.4	0.0	0.1
10c	0.4	0.7	60	29
10d	0.4	0.2	11	2
10e	1.7	0.7	52	6

¹ Percentage of remaining compound after 60 min of incubation with human and mouse microsomes obtained from Tebu-Xenotech in the presence of NADPH at 37 °C. See Materials and Methods for further details. The ² IC₅₀ values are the average of three replicates. The fluorescent assay as performed here has a standard error between 10 and 20% suggesting that differences of two-fold or greater are significant. Because of the limitations of the assay, it is difficult to distinguish among potencies < 0.5 nM [28].