Table 1.
Data and specimens collected.
| Data Sources | Description | Purpose |
|---|---|---|
| Environmental and occupational exposure questionnaire | Environmental exposures, source of drinking water, organic eating behaviors, residence history, occupation. | Gather socio-demographic data and understand the occupational exposure of farmers. |
| Maternal health history questionnaire | Personal medical history, reproductive history, pregnancy status, family history of cancer, demographic data. | Gather socio-demographic data and identify any health risks on pregnancy. |
| Food Frequency questionnaire | Summary of dietary intake frequencies over the previous year. | Usual food consumption, including portion sizes, during the previous 12 months. Nutrient and food group intake estimates. |
| Dietary recalls | Complete report of all food, drink, and supplements consumed the previous day (3 recalls were requested). | Validate data reported on the FFQ and reduce the risk of bias. |
| Specimens collected | Description | Purpose |
| Urine | First-void urine samples | Biomonitoring of pesticides metabolites |
| Blood | Peripheral blood, separated into serum for biochemical analysis and biomonitoring, and whole blood for DNA extraction. It includes blood from farmers, maternal blood prior to and during delivery, and cord blood. | Biomonitoring. Complementary biochemical analysis. Oxidative stress biomarkers quantification. DNA Methylation. |
| Hair | Optional sample | Biomonitoring of pesticides. |
| Buccal cells | Optional sample, collected from infants in the cohort study. | DNA extraction. Biomarkers identification. Epigenetics. |
| Meconium | 2 stools of the baby. | Biomonitoring of pesticides. |