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. 2021 Dec 7;10(24):5731. doi: 10.3390/jcm10245731

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Label-free Nanoflow UHPLC-MS/MS analytical workflow for the proteomic analysis of human aqueous humor. Samples were digested using trypsin and were analyzed using an LTQ-Orbitrap DiscoveryTM hybrid mass spectrometer (Thermo Electron). Proteins were identified and quantified using the SEQUEST algorithm followed by analysis using Xcalibur 2.0 SR1 (Thermo Electron). The intersection of each area represents the number of significant expression (p < 0.05) proteins between each groups. Only one protein was significantly deferentially expressed in each group.