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. 2021 Dec 16;9(12):1491. doi: 10.3390/vaccines9121491

Figure 1.

Figure 1

Figure 1

BHK-21 cells transfected with pSinCCHF-52S encoding His-tagged CCHFV nucleoprotein (NP) fused to the polyhistidine tag were specifically stained with anti-His6 antibody (A,B) and anti-CCHFV IgG human serum (C,D) in immunofluorescence test, and expressed protein with a molecular mass of 52 kDa stained with anti-His tag antibodies (E). BHK-21 cells transfected with replicon pSinCCHF-52S (A,C); mock-transfected BHK-21 cells (B,D); images were captured using the Olympus BX51 fluorescence microscope (USA) (×40). Western-blot analysis of CCHFV NP using mouse anti-His6 monoclonal antibody (E): Lane 1: MagicMark XP Western Protein Standard, Lane 2: BHK-21 cells transfected with pSinCCHF-52S, Lane 3: Mock-transfected BHK-21 cells. The position of the molecular mass marker is shown on the left.