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Kinetics of CCHFV NP total RNA expression in BHK-21 cells electroporated with pSinCCHF-52S registered 4 to 48 h post-transfection. No CCHFV RNA was detected in cells after transfection with pSinGFP. * Signal was significantly higher than that generated by qRT-PCR of cells transfected with pSinGFP (<1.0 × 103; p < 0.05; Supplementary Table S1).
