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. 2021 Dec 16;9(12):1491. doi: 10.3390/vaccines9121491

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Antibody responses induced by immunization with pSinCCHF-52S. Indirect immunofluorescence analysis of serum CCHFV NP IgG antibodies from pSinCCHF-52S immunized mice with and without Poly (I:C) (A1–A5): (A1) mouse 1, (A2) mouse 2, (A3) mouse 3, (A4) mouse 4, (A5) mouse 5 compared to mice immunized with pSinGFP (B1–B3): (B1) mouse 1, (B2) mouse 2, and (B3) mouse 3. (C1), positive control. Images were captured using the Nikon ECLIPSE Ni-U fluorescence microscope (USA) (×20). Anti-CCHFV NP IgG endpoint titer (C). Anti-CCHFV NP IgG endpoint titer and IgG2a/IgG1 ratios (D). Mice (NIH; n = 5/group) were immunized three times intramuscularly with the prepared pSinCCHF-52S construct expressing CCHFV nucleoprotein with (n = 2) and without Poly (I:C) (n = 3). Serum anti-CCHFV NP IgG were analyzed using a commercially available indirect immunofluorescent assay. Data are expressed as the mean for five mice (pSinCCHF-52S) and three mice for pSinGFP and the standard error of the mean. * p < 0.05 by Mann–Whitney U test.